it is possible but not a guarantee. It depends on what happens to sales over the long run and exactly how much they were spending on advertising. If they spent a large sum of money and it wasn't very effective advertising they might not lose that much sales and profits might actually go up.
If you don't know what the product or service is that the business is selling would you spend money there? Advertising dramatically increases awareness which translates into sales.
Yes it's very obvious they would lose money if they stopped advertising!
If they have no competition and are the sole supplier of their product, then advertising may not be as essential, but in competitive fields and products with limited inherent value like some supplements and sugar water their sales are clearly the product of their advertising.
Maybe, maybe not. It all depends.